目的 研究桐叶千金藤(Stephania hernandifolia Walp.)中的化学成分。方法 桐叶千金藤全草用体积分数95%乙醇溶液加热回流提取,减压回收乙醇,浓缩液加水稀释后先用HCl调节pH值,石油醚萃取,再经碱处理,石油醚、三氯甲烷萃取,得总生物碱。总生物碱利用正相硅胶、反相Rp-18、微孔吸附树脂CHP 20P、Sephadex LH-20等柱色谱方法分离纯化,通过理化性质和光谱数据进行结构鉴定。结果 从桐叶千金藤中分离得到1个氧化异阿朴啡型生物碱:9-O-去甲基蝙蝠葛宁(1)。结论 化合物1为一新的化合物。
Abstract
OBJECTIVE To study the chemical constituents of Stephania hernandifolia.METHODS The dried herbs of S. hernandifolia were extracted with 95% ethanol. After removal of the solvent, the residue was first partitioned between acid water and petroleum ether, then the aqueous layer was basified and extracted with chloroform to obtain crude alkaloids. Column chromatograghy on silica gel, Rp-18, MCI CHP 20P, and Sephadex LH-20 were applied for the isolation and purification of the crude alkaloids fraction. The structures were elucidated by their physicochemical properties and spectral data. RESULTS One oxoisoaporphine-type alkaloid, 9-O-demethylbianfugenine(1) was isolated from the whole plants of S. hernandifolia.CONCLUSION Compound 1 is a new compound.
关键词
氧化异阿朴啡型生物碱 /
9-O-去甲基蝙蝠葛宁 /
蝙蝠葛宁 /
防己科 /
桐叶千金藤
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Key words
oxoisoaporphine-type alkaloid /
9-O-demethylbianfugenine /
bianfugenine /
Menispermaceae /
Stephania hernandifolia
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中图分类号：
R284
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参考文献
[1] State Administration of Traditional Chinese Medicine. Zhonghua Bencao(中华本草第八卷)[M]. Vol 8, Shanghai: Scentific and Technical Publishing House,1999:203-204.[2] SEMWAL D K, BADONI R, SEMWAL R, et al. The genus Stephania(Menispermaceae):Chemical and pharmacological perspectives [J]. J Ethnopharm,2010, 132(2):369-383.[3] WANG X K, ZHAO T F. The alkaloids of Stephania [J]. West China J Pharm Sci(华西药学杂志), 1990, 5(2):87-93.[4] KUPCHAN S M, SUFFNESS M I, WHITE D N J, et al. Isolation and structural elucidation of 4-demethylhasubanonine, a new alkaloid from Stephania hernandifolia [J]. J Org Chem, 1968, 33(12):4529-4532.[5] TANG L J, ZHANG Y H, HAO X Y, et al. Hasubanan type alkaloids in Stephania hernandifolia [J]. China J Chin Mater Med (中国中药杂志), 2010, 35(15):1973-1977.[6] TANG L J, GUAN H Y, ZHANG Y H, et al. Study on the alkaloids in Stephania hernandifolia [J]. J Chin Med Mater (中药材), 2010, 33(12):1881-1883.[7] TANG L J, DENG L L, HE L, et al. Three new hasubanan alkaloids from Stephania hernandifolia (Willd.) Walp [J]. Helv Chim Acta, 2013, 96(10):1930-1935.[8] HUANG Z Y, ZHANG Y H, ZHOU L, et al. Chemical constituents of Sinomenium acutum [J]. Chin Tradit Herb Drugs (中草药), 2009, 40(2):193-196.[9] TAKANI M, TAKASU Y, TAKAHASHI K. Studies on constituents of medicinal plants. XXIII.Constituents of the vines of Menispermum dauricum DC.(2) [J]. Chem Pharm Bull, 1983, 31(9):3091-3093.
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脚注
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基金
科技部973计划前期研究专项项目(2012CB722601); 国家重大科学仪器设备开发专项项目(2011YQ12003506)
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